Cross-species amplification of microsatellite loci in aphids: assessment and application

Wilson, ACC; Massonnet, B; Simon, JC; Prunier-Leterme, N; Dolatti, L; Llewellyn, KS; Figueroa, CC; Ramirez, CC; Blackman, RL; Estoup A.; SUNNUCKS, P.

Abstract

Despite the relative ease of isolating microsatellites, their development still requires substantial inputs of time, money and expertise. For this reason there is considerable interest in using existing microsatellites on species from which markers were not cloned. We tested cross-species amplification of 48 existing aphid loci in species of the following genera: Aphidinae: Aphidini: Aphis and Rhopalosiphum; Aphidinae: Macrosiphini: Acyrthosiphum, Brevicoryne, Diuraphis, Illinoia, Macrosiphoniella, Macrosiphum, Metopeurum, Metapolophium, Myzus, Phorodon, Sitobion and Uroleucon and Neuquenaphidinae: Neuquenaphis. Our results show cross-species application of known microsatellite loci is a highly promising source of codominant markers for population genetic and evolutionary studies in aphids.

Más información

Título según WOS: Cross-species amplification of microsatellite loci in aphids: assessment and application
Título según SCOPUS: Cross-species amplification of microsatellite loci in aphids: Assessment and application
Título de la Revista: MOLECULAR ECOLOGY NOTES
Volumen: 4
Número: 1
Editorial: WILEY-BLACKWELL
Fecha de publicación: 2004
Página de inicio: 104
Página final: 109
Idioma: English
DOI:

10.1046/j.1471-8286.2003.00584.x

Notas: ISI, SCOPUS