Abstract

In the present work, we describe the synthesis of zinc oxide nanoparti-des (ZnO NPs) using culture supernatant of endophytic bacterial isolate Sphingobacterium thalpophilum and their antibacterial efficiency against bacterial pathogens. In the process of reduction aqueous zinc nitrate being extra-cellular which lead to the development of an easy bioprocess for synthesis of ZnO NPs. The ZnO NPs were characterized by X-ray Powder Diffraction (XRD), Field emission scanning electron microscopy (FESEM) and Energy dispersive X-ray analysis (EDAX). Ultraviolet-Visible (UV-Vis) Spectrometer and Fourier transfer infrared rays (FTIR) analysis. Antibacterial efficiency of ZnO NPs are tested with two bacterial pathogens Pseudomonas aeruginosa and Enterobacter aerogens using the disc diffusion method to determine their ability as potential antimicrobial agents. This ZnO NPs showed improved antibacterial activity on both tested strains. This was confirmed by zone of inhibition measurements.