Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems
Abstract
Aims: Test the use of nondegenerated consensus polymerase chain reaction (PCR) primers targeting lip and mnp sequences to detect ligninolytic fungi in wood-decaying soil systems, avoiding the need for enrichment or isolation on traditional fungal media culture. Methods and Results: The PCR primers were tested with total DNA isolated from incubations of wood-soil systems inoculated or not with the white-rot fungi Phanerochaete chrysosporium, or a white-rot sample obtained from a Nothofagus forest. The PCR products for lip and mnp sequences were only obtained in soil with P. chrysosporium-colonized wood chips. In these soil samples, reverse transcription-PCR analysis of lip and mnp PCR products indicated expression of LipA, LipB, LipJ and MnP isoenzymes. Significance and Impact of the Study: This is the first assessment of the use of consensus PCR primers for direct detection of ligninolytic peroxidase genes in wood-decaying soil systems.
Más información
Título según WOS: | Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems |
Título según SCOPUS: | Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems |
Título de la Revista: | LETTERS IN APPLIED MICROBIOLOGY |
Volumen: | 38 |
Número: | 1 |
Editorial: | Wiley |
Fecha de publicación: | 2004 |
Página de inicio: | 43 |
Página final: | 49 |
Idioma: | English |
DOI: |
10.1046/j.1472-765X.2003.01442.x |
Notas: | ISI, SCOPUS |