Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems

Stuardo, M.; Vásquez M; VICUNA, R; Gonzalez, B.

Abstract

Aims: Test the use of nondegenerated consensus polymerase chain reaction (PCR) primers targeting lip and mnp sequences to detect ligninolytic fungi in wood-decaying soil systems, avoiding the need for enrichment or isolation on traditional fungal media culture. Methods and Results: The PCR primers were tested with total DNA isolated from incubations of wood-soil systems inoculated or not with the white-rot fungi Phanerochaete chrysosporium, or a white-rot sample obtained from a Nothofagus forest. The PCR products for lip and mnp sequences were only obtained in soil with P. chrysosporium-colonized wood chips. In these soil samples, reverse transcription-PCR analysis of lip and mnp PCR products indicated expression of LipA, LipB, LipJ and MnP isoenzymes. Significance and Impact of the Study: This is the first assessment of the use of consensus PCR primers for direct detection of ligninolytic peroxidase genes in wood-decaying soil systems.

Más información

Título según WOS: Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems
Título según SCOPUS: Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems
Título de la Revista: LETTERS IN APPLIED MICROBIOLOGY
Volumen: 38
Número: 1
Editorial: Wiley
Fecha de publicación: 2004
Página de inicio: 43
Página final: 49
Idioma: English
DOI:

10.1046/j.1472-765X.2003.01442.x

Notas: ISI, SCOPUS