MCUR1 Is a Scaffold Factor for the MCU Complex Function and Promotes Mitochondrial Bioenergetics

Tomar, Dhanendra; Dong, Zhiwei; Shanmughapriya, Santhanam; Koch, Diana A.; Thomas, Toby; Hoffman, Nicholas E.; Timbalia, Shrishiv A.; Goldman, Samuel J.; Breves, Sarah L.; Corbally, Daniel P.; Nemani, Neeharika; Fairweather, Joseph P.; Cutri, Allison R.; Zhang, Xueqian; Song, Jianliang; et. al.

Abstract

Mitochondrial Ca2+ Uniporter (MCU)-dependent mitochondrial Ca2+ uptake is the primary mechanism for increasing matrix Ca2+ in most cell types. However, a limited understanding of the MCU complex assembly impedes the comprehension of the precise mechanisms underlying MCU activity. Here, we report that mouse cardiomyocytes and endothelial cells lacking MCU regulator 1 (MCUR1) have severely impaired [Ca2+](m) uptake and I-MCU current. MCUR1 binds to MCU and EMRE and function as a scaffold factor. Our protein binding analyses identified the minimal, highly conserved regions of coiled-coil domain of both MCU and MCUR1 that are necessary for heterooligomeric complex formation. Loss of MCUR1 perturbed MCU heterooligomeric complex and functions as a scaffold factor for the assembly of MCU complex. Vascular endothelial deletion of MCU and MCUR1 impaired mitochondrial bioenergetics, cell proliferation, and migration but elicited autophagy. These studies establish the existence of a MCU complex that assembles at the mitochondrial integral membrane and regulates Ca2+-dependent mitochondrial metabolism.

Más información

Título según WOS: ID WOS:000376654500006 Not found in local WOS DB
Título de la Revista: CELL REPORTS
Volumen: 15
Número: 8
Editorial: Cell Press
Fecha de publicación: 2016
Página de inicio: 1673
Página final: 1685
DOI:

10.1016/j.celrep.2016.04.050

Notas: ISI