Pseudomonas cuatrocienegasensis sp nov., isolated from an evaporating lagoon in the Cuatro Cienegas valley in Coahuila, Mexico

Escalante, Ana E.; Caballero-Mellado, Jesus; Martinez-Aguilar, Lourdes; Rodriguez-Verdugo, Alejandra; Gonzalez-Gonzalez, Andrea; Toribio-Jimenez, Jeiry; Souza, Valeria

Abstract

Nine Gram-negative, rod-shaped, non-spore-forming isolates with identical or very similar repetitive-sequence-based PCR profiles were recovered from an evaporative lagoon in Mexico. Two strains, designated 1N(T) and 3N, had virtually identical 16S rRNA gene sequences and, on the basis of these sequences, were identified as members of the genus Pseudomonas, with Pseudomonas peli R-20805(T) as the closest relative. All nine isolates had practically identical whole-cell protein profiles. The major fatty acids [C-16:0, C-18:1 omega 7c and summed feature a (C-16:1 omega 7 and/or C-16:1 omega 6c)] of strains 1N(T) and 3N supported their affiliation with the genus Pseudomonas. The DNA-DNA reassociation values with respect to P. peli LMG 23201(T) and other closely related Pseudomonas species were 15 %. Physiological and biochemical tests allowed phenotypic differentiation of the strains analysed, including strain 1 IN, from the five phylogenetically closest Pseudomonas species. On the basis of the data obtained by using this polyphasic: taxonomic approach, the nine strains represent a novel species, for which the name Pseudomonas cuatrocienegasensis sp. nov. is proposed. The type strain is 1N(T) (=LMG 24676(T)=CIP 109853(T)).

Más información

Título según WOS: ID WOS:000267645000029 Not found in local WOS DB
Título de la Revista: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volumen: 59
Editorial: MICROBIOLOGY SOC
Fecha de publicación: 2009
Página de inicio: 1416
Página final: 1420
DOI:

10.1099/ijs.0.006189-0

Notas: ISI