Recombinant expression of ShPI-1A, a non-specific BPTI-Kunitz-type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris

Gil, Dayrom F.; Garcia-Fernandez, Rossana; Alonso-del-Rivero, Maday; Lamazares, Emilio; Perez, Mariela; Varas, Laura; Diaz, Joaquin; Chavez, Maria A.; Gonzalez-Gonzalez, Yamile; Mansur, Manuel

Abstract

Pichia pastoris is a highly successful system for the large-scale expression of heterologous proteins, with the added capability of performing most eukaryotic post-translational modifications. However, this system has one significant disadvantage - frequent proteolytic degradation by P. pastoris proteases of heterologously expressed proteins. Several methods have been proposed to address this problem, but none has proven fully effective. We tested the effectiveness of a broad specificity protease inhibitor to control proteolysis. A recombinant variant of the BPTI-Kunitz protease inhibitor ShPI-1 isolated from the sea anemone Stichodactyla helianthus, was expressed in P. pastoris. The recombinant inhibitor (rShPI-1A), containing four additional amino acids (EAEA) at the N-terminus, was folded similarly to the natural inhibitor, as assessed by circular dichroism. rShPI-1A had broad protease specificity, inhibiting serine, aspartic, and cysteine proteases similarly to the natural inhibitor. rShPI-1A protected a model protein, recombinant human miniproinsulin (rhMPI), from proteolytic degradation during expression in P. pastoris. The addition of purified rShPI-1A at the beginning of the induction phase significantly protected rhMPI from proteolysis in culture broth. The results suggest that a broad specificity protease inhibitor such as rShPI-1A can be used to improve the yield of recombinant proteins secreted from P. pastoris.

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Título según WOS: ID WOS:000296398100005 Not found in local WOS DB
Título de la Revista: FEMS YEAST RESEARCH
Volumen: 11
Número: 7
Editorial: OXFORD UNIV PRESS
Fecha de publicación: 2011
Página de inicio: 575
Página final: 586
DOI:

10.1111/j.1567-1364.2011.00749.x

Notas: ISI