Identification and characterization of novel factors that act in the nonsense-mediated mRNA decay pathway in nematodes, flies and mammals

Casadio, Angela; Longman, Dasa; Hug, Nele; Delavaine, Laurent; Baier, Raul Vallejos; Alonso, Claudio R.; Caceres, Javier F.

Abstract

Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome-wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp-1) and SEC13 (npp-20), are also required for NMD in human cells. We also show that the C. elegans gene noah-2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process.

Más información

Título según WOS: ID WOS:000347304500010 Not found in local WOS DB
Título de la Revista: EMBO REPORTS
Volumen: 16
Número: 1
Editorial: Wiley
Fecha de publicación: 2015
Página de inicio: 71
Página final: 78
DOI:

10.15252/embr.201439183

Notas: ISI