FIELD PERFORMANCE OF CRYOPRESERVED SEED-DERIVED MAIZE PLANTS
Keywords: recovery, genetic integrity, SHOOT-TIPS, DROPLET-VITRIFICATION
Abstract
Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks. OBJECTIVE: To describe the field performance of cryopreserved seed - derived maize adult plants. MATERIALS AND METHODS: Germination percentage and numbers of leaves and ears per plant, internodes in stems, middle - aged leaf length, plant height, ear traits and weight of 100 seeds were recorded. RESULTS: Statistically significant differences between adult plants derived from cryopreserved seeds and the control treatment were not observed (t-test, p=0.05). CONCLUSION: The results presented confirm at the phenotype level the effectiveness of maize seed cryostorage to preserve and regenerate true-to-type plants.
Más información
Título de la Revista: | CRYOLETTERS |
Volumen: | 39 |
Número: | 6 |
Editorial: | CRYO LETTERS, C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND |
Fecha de publicación: | 2018 |
Página de inicio: | 366 |
Página final: | 370 |
Idioma: | English |
Financiamiento/Sponsor: | Cuban Ministry for Superior Education |
URL: | https://www.ingentaconnect.com/content/cryo/cryo/2018/00000039/00000006/art00004 |