FIELD PERFORMANCE OF CRYOPRESERVED SEED-DERIVED MAIZE PLANTS

Arguedas, Melissa; Villalobos, Ariel; Gomez, Daviel; Hernandez, Lazaro; Zevallos, Byron E.; Cejas, Inaudis; Yabor, Lourdes; Edel Martinez-Montero, Marcos; Lorenzo, Jose Carlos

Keywords: recovery, genetic integrity, SHOOT-TIPS, DROPLET-VITRIFICATION

Abstract

Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks. OBJECTIVE: To describe the field performance of cryopreserved seed - derived maize adult plants. MATERIALS AND METHODS: Germination percentage and numbers of leaves and ears per plant, internodes in stems, middle - aged leaf length, plant height, ear traits and weight of 100 seeds were recorded. RESULTS: Statistically significant differences between adult plants derived from cryopreserved seeds and the control treatment were not observed (t-test, p=0.05). CONCLUSION: The results presented confirm at the phenotype level the effectiveness of maize seed cryostorage to preserve and regenerate true-to-type plants.

Más información

Título de la Revista: CRYOLETTERS
Volumen: 39
Número: 6
Editorial: CRYO LETTERS, C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND
Fecha de publicación: 2018
Página de inicio: 366
Página final: 370
Idioma: English
Financiamiento/Sponsor: Cuban Ministry for Superior Education
URL: https://www.ingentaconnect.com/content/cryo/cryo/2018/00000039/00000006/art00004