A choline-evoked [Ca2+](C) signal causes catecholamine release and hyperpolarization of chromaffin cells

Fuentealba, J; Olivares, R; Ales, E; Tapia, L; Rojo, J; Arroyo, G; Aldea, M; Criado, M; Gandia, L; Garcia, AG

Abstract

In bovine chromaffin cells fast-superfused with Krebs-HEPES solution containing 1 - 2 mM Ca2+, 5 s pulses of choline ( 1 - 10 mM), elicited catecholamine secretory responses that were only similar to10% of those evoked by ACh (0.01 - 0.1 mM). However, in high-Ca2+ solutions (10 - 20 mM) the size of the choline secretory responses approached those of ACh. The choline responses ( 10 mM choline in 20 mM Ca2+, 10Cho/20Ca(2+)) tended to decline upon repetitive pulsing, whereas those of ACh were well maintained. The confocal [Ca2+](c) increases evoked by 10Cho/20Ca(2+) were similar to those of ACh. Whereas 10Cho/20Ca(2+) caused mostly hyperpolarization of chromaffin cells, 0.1 ACh/20 Ca2+ caused first depolarization and then hyperpolarization; in regular solutions (2 mM Ca2+), the hyperpolarizing responses did not show up. In Xenopus oocytes injected with mRNA for bovine alpha7 nicotinic receptors (nAChRs), 10Cho/20 Ca2+ fully activated an inward current; in oocytes expressing alpha3beta4, however, the inward current elicited by choline amounted to only 4% of the size of alpha7 current. Our results suggest that choline activates the entry of Ca2+ through a7 nAChRs; this leads to a cytosolic concentration of calcium ([Ca2+](c)) rise that causes the activation of nearby Ca2+-dependent K+ channels and the hyperpolarization of the chromaffin cell. This response, which could be unmasked provided that cells were stimulated with high-Ca2+ solutions, may be the underlying mechanism through which choline exerts a modulatory effect on the electrical activity of the chromaffin cell and on neurotransmitter release at cholinergic synapses.

Más información

Título según WOS: ID WOS:000222979200014 Not found in local WOS DB
Título de la Revista: FASEB JOURNAL
Volumen: 18
Número: 10
Editorial: Wiley
Fecha de publicación: 2004
Página de inicio: 1468
Página final: +
DOI:

10.1096/fj.04-1828fje

Notas: ISI