Conserved genomic collinearity as a source of broadly applicable, fast evolving, markers to resolve species complexes: A case study using the lichen -forming genus Peltigera section Polydactylon

Magain, Nicolas; Miadlikowska, Jolanta; Mueller, Olaf; Gajdeczka, Michael; Truong, Camille; Salamov, Asaf A.; Dubchak, Inna; Grigoriev, Igor V.; Goffinet, Bernard; Serusiaux, Emmanuel; Lutzoni, Francois

Abstract

Synteny can be maintained for certain genomic regions across broad phylogenetic groups. In these homologous genomic regions, sites that are under relaxed purifying selection, such as intergenic regions, could be used broadly as markers for population genetic and phylogenetic studies on species complexes. To explore the potential of this approach, we found 125 Collinear Orthologous Regions (COR) ranging from 1 to > 10 kb across nine genomes representing the Lecanoromycetes and Eurotiomycetes (Pezizomycotina, Ascomycota). Twenty-six of these COR were found in all 24 eurotiomycete genomes surveyed for this study. Given the high abundance and availability of fungal genomes we believe this approach could be adopted for other large groups of fungi outside the Pezizomycotina. As a proof of concept, we selected three Collinear Orthologous Regions (COR1b, COR3, and COR16), based on synteny analyses of several genomes representing three classes of Ascomycota: Eurotiomycetes, Lecanoromycetes, and Lichinomycetes. COR16, for example, was found across these three classes of fungi. Here we compare the resolving power of these three new markers with five loci commonly used in phylogenetic studies of fungi, using section Polydactylon of the cyanolichen-forming genus Peltigera (Lecanoromycetes) - a Glade with several challenging species complexes. Sequence data were subjected to three species discovery and two validating methods. COR markers substantially increased phylogenetic resolution and confidence, and highly contributed to species delimitation. The level of phylogenetic signal provided by each of the COR markers was higher than the commonly used fungal barcode ITS. High cryptic diversity was revealed by all methods. As redefined here, most species represent lineages that have relatively narrower, and more homogeneous biogeographical ranges than previously understood. The scabrosoid dade consists of ten species, seven of which are new. For the dolichorhizoid Glade, twenty-two new species were discovered for a total of twenty-nine species in this dade.

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Título según WOS: ID WOS:000417011500003 Not found in local WOS DB
Título de la Revista: MOLECULAR PHYLOGENETICS AND EVOLUTION
Volumen: 117
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2017
Página de inicio: 10
Página final: 29
DOI:

10.1016/j.ympev.2017.08.013

Notas: ISI