Brain acetylcholinesterase promotes amyloid-beta-peptide aggregation but does not hydrolyze amyloid precursor protein peptides

Campos, EO; Alvarez, A; Inestrosa, NC

Abstract

It has been suggested that acetylcholinesterase (AChE) has both a putative proteolytic activity against the amyloid precursor protein (APP), and a capacity to accelerate the assembly of amyloid-beta-peptide (A beta) into Alzheimer's fibrils. Here, we have studied the ability of bovine brain AChE to share both activities. Results indicate that AChE purified through acridinium was able to process the APP peptides, however after further purification by an edrophonium column, the protease activity was lost. Under both conditions the capacity of the enzyme to promote amyloid formation was maintained. Kinetic studies of the A beta aggregation process using edrophonium-AChE, indicated that the lag phase of the aggregation process was smaller than the one observed with the esterase purified by acridinium alone. Considering that the total amount of amyloid formed, measured by thioflavine-T fluorescence, was similar for both AChE preparations, our results suggest that the edrophonium-AChE possesses an higher intrinsic capacity to stimulate the aggregation of A beta(1-40) peptide.

Más información

Título según WOS: ID WOS:000071739700003 Not found in local WOS DB
Título de la Revista: NEUROCHEMICAL RESEARCH
Volumen: 23
Número: 2
Editorial: SPRINGER/PLENUM PUBLISHERS
Fecha de publicación: 1998
Página de inicio: 135
Página final: 140
DOI:

10.1023/A:1022416505725

Notas: ISI