EARLY AXONAL REGENERATION - REPRESSION BY SCHWANN-CELLS AND A PROTEASE

TAPIA, M; INESTROSA, NC; ALVAREZ, J

Abstract

We have proposed that mature Schwann cells and an extracellular protease repress the sprouting response of axons. To test this hypothesis, we destroyed all cells by freezing a short span of the rat sciatic nerve or inhibited proteases with subperineurial injections of aprotinin, and a crush was made to induce the sprouting response. In unconditioned or in vehicle-injected nerves, axons began to elongate at a constant rate after a delay of about 1 day. The freezing of the nerve distal to the crush obliterated the delay, but the rate of elongation did not change. A similar pattern was observed when the nerve segment was conditioned with aprotinin for 2 days prior to the crush. These effects were abolished when a short untreated segment was left between crush and conditioned region of the nerve. The electron microscopy of the nerve and the immunolocalization of the growth-associated protein (GAP-43) were consistent with the enhanced regrowth observed in conditioned nerves. Our findings support the notion that Schwann cells repress the onset of regeneration and that a local protease is involved. (C) 1995 Academic Press, Inc.

Más información

Título según WOS: ID WOS:A1995QN05600013 Not found in local WOS DB
Título de la Revista: EXPERIMENTAL NEUROLOGY
Volumen: 131
Número: 1
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 1995
Página de inicio: 124
Página final: 132
DOI:

10.1016/0014-4886(95)90014-4

Notas: ISI