Article ISI
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS  (2020)

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Stuardo, Nicolas; Moya-Alvarado, Guillermo; RAMIREZ, CAROLINA; Schiavo, Giampietro; Bronfman, Francisca C.

Abstract

Recombinant BDNF containing an Avi sequence (BDNFAvi) is produced in HEK293 cells and then cost-effectively purified by affinity chromatography. A reproducible protocol was developed to directly mono-biotinylate BDNFAvi with the enzyme BirA in a tube. In this reaction, mono-biotinylated BDNFAvi retains its biological activity.

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Título según WOS: An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
Título de la Revista: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Número: 161
Editorial: JOURNAL OF VISUALIZED EXPERIMENTS
Fecha de publicación: 2020
DOI:

10.3791/61262
Notas: ISI