A hot-sensing cold receptor: C-terminal domain determines thermosensation in transient receptor potential channels

Brauchi, S.; Orta, G; Salazar M.; Rosenmann, E; Latorre R.

Abstract

Temperature transduction inmammalsis possible because of the presence of a set of temperature-dependent transient receptor potential (TRP) channels in dorsal root ganglia neurons and skin cells. Six thermo-TRP channels, all characterized by their unusually high temperature sensitivity (Q10 > 10), have been cloned: TRPV1-4 are heat activated, whereas TRPM8 and TRPA1 are activated by cold. Because of the lack of structural information, the molecular basis for regulation by temperature remains unknown. In this study, we assessed the role of the C-terminal domain of thermo-TRPs and its involvement in thermal activation by using chimeras between the heat receptor TRPV1 and the cold receptor TRPM8, in which the entire C-terminal domain was switched. Here, we demonstrate that the C-terminal domain is modular and confers the channel phenotype regarding temperature sensitivity, channel gating kinetics, and PIP2 (phosphatidylinositol-4,5-bisphophate) modulation. Thus, thermo-TRP channels contain an interchangeable specific region, different from the voltage sensor, which allows them to sense temperature stimuli. Copyright © 2006 Society for Neuroscience.

Más información

Título según WOS: A hot-sensing cold receptor: C-terminal domain determines thermosensation in transient receptor potential channels
Título según SCOPUS: A hot-sensing cold receptor: C-terminal domain determines thermosensation in transient receptor potential channels
Título de la Revista: JOURNAL OF NEUROSCIENCE
Volumen: 26
Número: 18
Editorial: SOC NEUROSCIENCE
Fecha de publicación: 2006
Página de inicio: 4835
Página final: 4840
Idioma: English
URL: http://www.jneurosci.org/cgi/doi/10.1523/JNEUROSCI.5080-05.2006
DOI:

10.1523/JNEUROSCI.5080-05.2006

Notas: ISI, SCOPUS