Effects of long-term adrenalectomy on apoptosis and neuroprotection in the rat hippocampus

Andres S; Cardenas, S; Parra C.; Bravo, J.; Greiner M.; Rojas P.; Morales P.; Lara, H; Fiedler J.

Abstract

Reduction in corticosterone by acute adrenalectomy (5 d) promotes apoptosis in dentate gyrus (DG) granular neurons, an effect concomitant with variations in the expression of the Bcl-2 gene family implicated in apoptotic regulation. However, no studies exist correlating the effect of long-term adrenalectomy (30 d) on the hippocampus in terms of extent of apoptosis and the levels of proteins related to an apoptotic cascade. After 5 d of adrenalectomy, we found an increase in apoptosis of the DG granular region, correlated with an increase in the processing of caspase-9. The magnitude of apoptosis 30 d after adrenalectomy was reduced in the DG granular layer compared with 5 d after adrenalectomy, in close relation to a reduction in the level of processed caspase-9. To understand how the increase in cell survival long after adrenalectomy occurs, we analyzed changes in the expression of genes and proteins related to apoptosis. Long-term adrenalectomy did not change hippocampal pro-apoptotic Bax or antiapoptotic Bcl-2 mRNA levels or protein content with respect to control. However, we found an increase in mRNA levels of the GD's Bcl-x gene, in parallel with the increase in anti-apoptotic BCL-XL protein levels. These results suggest the reduction in apoptosis observed after long-term adrenalectomy occurs through mechanisms that repress proapoptotic genes previously found to be increased at shorter times of adrenalectomy. © 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.

Más información

Título según WOS: Effects of long-term adrenalectomy on apoptosis and neuroprotection in the rat hippocampus
Título según SCOPUS: Effects of long-term adrenalectomy on apoptosis and neuroprotection in the rat hippocampus
Título de la Revista: ENDOCRINE
Volumen: 29
Número: 2
Editorial: Humana Press, Inc.
Fecha de publicación: 2006
Página de inicio: 299
Página final: 307
Idioma: English
URL: http://link.springer.com/10.1385/ENDO:29:2:299
DOI:

10.1385/ENDO:29:2:299

Notas: ISI, SCOPUS