Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures
Abstract
The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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Título según WOS: | Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures |
Título según SCOPUS: | Expression of the ubiE gene of Geobacillus stearothermophilus V in Escherichia coli K-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures |
Título de la Revista: | APPLIED AND ENVIRONMENTAL MICROBIOLOGY |
Volumen: | 72 |
Número: | 1 |
Editorial: | AMER SOC MICROBIOLOGY |
Fecha de publicación: | 2006 |
Página de inicio: | 963 |
Página final: | 967 |
Idioma: | English |
URL: | http://aem.asm.org/cgi/doi/10.1128/AEM.72.1.963-967.2006 |
DOI: |
10.1128/AEM.72.1.963-967.2006 |
Notas: | ISI, SCOPUS |