Oxidative and nitrosative stress in frozen-thawed pig spermatozoa. II: Effect of the addition of saccharides to freezing medium on sperm function

Pezo, Felipe; Zambrano, Fabiola; Uribe, Pamela; Risopatron, Jennie; Moya, Claudia; Cesar de Andrade, Andre Furugen; Burgos, Rafael A.; Yeste, Marc; Sanchez, Raul

Abstract

Saccharides have bioprotective properties, with a high capacity to preserve biological proteins and membranes during sperm cryopreservation. The aim of this study was to evaluate how replacing the lactose of cryopreservation media by sucrose (SUC) or trehalose (TRE) at concentrations of 0.2 M (SUC-1 and TRE-1) and 0.25 M (SUC-2 and TRE-2) affects frozen/thawed pig spermatozoa. The media used were composed of medium A (saccharide/egg yolk) and B (saccharide/egg yolk/glycerol), their osmolality being determined prior to freezing. Cell viability, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), lipid peroxidation, thiol group oxidation, total reactive oxygen species (ROS), peroxynitrite and superoxide anion (O-2(center dot-)) were determined through flow cytometry; total motility (TM), progressive motility (PM) and kinetic parameters motility were determined immediately after thawing (T0) and again 30 (T30) and 60 (T60) minutes later. The SUC-2 and TRE-2 groups maintained viability significantly and presented fewer lipid membrane disorders, respectively, both with a significant increase in MMP. The production of O-2(center dot-) and peroxynitrite was lower in the TRE-2 groups compared to the control (P 0.05). Total motility at T0 was greater in the TRE-2 group (P 0.05). Sperm kinetics was not affected by the treatment. The use of saccharides SUC and TRE at a concentration of 0.25 M improves sperm quality, so that both non-penetrating cryoprotectants can be utilized in pig sperm freezing media.

Más información

Título según WOS: Oxidative and nitrosative stress in frozen-thawed pig spermatozoa. II: Effect of the addition of saccharides to freezing medium on sperm function
Título de la Revista: CRYOBIOLOGY
Volumen: 97
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2020
Página de inicio: 5
Página final: 11
DOI:

10.1016/j.cryobiol.2020.10.015

Notas: ISI