Abstract

Introduction: Exosomes are 40 to 100 nm-diameter vesicles released by cells and are formed within multivesicular bodies in the endosomal system. Normal human urine contains large numbers of exosomes, which are secreted by the epithelial cells of the urinary tract, and their isolation can result in marked enrichment of low abundance urinary proteins that have potential pathophysiologic significance. Previous studies from our laboratory have shown the kidney damage that in a model of streptozotocin-induced diabetic rat. Our current goal is to elucidate the putative protein markers involved in diabetic nephropathy by urinary exosomes isolation. Methodology: Urine was collect from control and diabetic rats with/without kidney damage and exosome isolation was performed through of ultracentrifugation, and the vesicles morphology were analyzed by electronic microscopic. For clinical biomarker discovery, LC-MS based large-scale quantitative proteomic analysis was realized. Results and conclusions: Diabetic nephropathy is the leading cause of kidney failure in Chile and the world. Therefore, it is necessary to identify new markers that are capable of detecting early stage for this disease. Our results show a significant difference in the protein content of healthy control rat-urine exosomes regarding early diabetic rat. In addition we could detect an increase in proteins isolated from urine exosomes diabetic rat of 8 months. These results suggest that urinary exosomes can be a useful tool in the identification of new markers associated with diabetic nephropathy (Innova-Corfo 13IDL2-23502)