Glycosylated narciclasine alkaloid in Hippeastrum puniceum (Lam.) Kuntze

Feu, Amanda Eiriz; de Andrade, Jean Paulo; Ayala, Alejandro Pedro; de Almeida, Larissa Costa; Costa-Lotufo, Leticia, V; Bastida, Jaume; Ellena, Javier; Borges, Warley de Souza

Abstract

The present work focused on the chemical study of the main alkaloid component in Brazilian indigenous Amaryllidaceae species Hippeastrum puniceum (Lam.) Kuntze. The strategy applied was based on a distinct partitioning process and chromatographic-purification steps using the crude methanolic extract as the starting material. In this way, the glycosylated derivative narciclasine-4-O-beta-D-xylopyranoside was isolated from the ethyl acetate fraction of H. puniceum. Structural elucidation of the compound was performed through NMR (Nuclear Magnetic Resonance) analysis including mono- and bi-dimensional experiments. The absolute configuration was determined herein for the first time by means of X-Ray Crystallography. The compound was also evaluated as cytotoxic agent against colon (HCT 116) and breast (MCF-7) tumor cells, showing no significant activity at the tested concentrations of 10 and 50 mu M. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.

Más información

Título según WOS: Glycosylated narciclasine alkaloid in Hippeastrum puniceum (Lam.) Kuntze
Título de la Revista: SOUTH AFRICAN JOURNAL OF BOTANY
Volumen: 136
Editorial: Elsevier
Fecha de publicación: 2021
Página de inicio: 30
Página final: 34
DOI:

10.1016/j.sajb.2020.09.006

Notas: ISI