Characterization of geographically isolated accessions in five Alstroemeria L. species (Chile) using FISH of tandemly repeated DNA sequences and RAPD analysis

Baeza, C.; Schrader O.; Budahn, H

Abstract

In fifteen geographically isolated populations of five species of Alstroemeria L. (A. aurea, A. hookeri, A. ligtu, A. pelegrina and A. presliana) collected in Chile, karyotypes and variation of RAPD markers were investigated. Tandemly repeated DNA sequences - 5S and 18/25S rDNA genes and the sequence A001-1 (De Jeu et al. 1997) were used to characterize karyotypes by fluorescence in situ hybridization (FISH). Ten somatic metaphases per population were used for measurement of chromosome length. Differences in RAPD marker bands were used for characterization of populations, creating a similarity index. FISH with all three DNA probes shows a high degree of polymorphism between and sometimes also within accessions of A. aurea, A. hookeri and A. ligtu. The number of chromosome pairs showing 5S rDNA signals is more different for the investigated species A. aurea, A. hookeri, A. ligtu, A. pelegrina and A. presliana with 5, 7, 5, 3 and 7, respectively, than the number of 18/25S rDNA signals in this succession with 7, 7, 6, 5 and 7 chromosome pairs, showing a high evolutionary dynamics within the genus. Furthermore, among the four populations of A. hookeri, accession 4181 was different in arm length of chromosome 3. RAPD markers (index of similarity) also showed a greater genetic distance of accession 4181 from the other three accessions of A. hookeri. The possible evolutionary mechanisms providing these polymorphisms were discussed. © 2007 Springer.

Más información

Título según WOS: Characterization of geographically isolated accessions in five Alstroemeria L. species (Chile) using FISH of tandemly repeated DNA sequences and RAPD analysis
Título según SCOPUS: Characterization of geographically isolated accessions in five Alstroemeria L. species (Chile) using FISH of tandemly repeated DNA sequences and RAPD analysis
Título de la Revista: PLANT SYSTEMATICS AND EVOLUTION
Volumen: 269
Número: 01-feb
Editorial: SPRINGER WIEN
Fecha de publicación: 2007
Página de inicio: 1
Página final: 14
Idioma: English
URL: http://link.springer.com/10.1007/s00606-007-0591-5
DOI:

10.1007/s00606-007-0591-5

Notas: ISI, SCOPUS