Abstract

Simple Summary: After administration of antimicrobials, poultry excrete significant concentrations of antimicrobials through their droppings, which accumulate in the litter where poultry are housed. Poultry litter, which consists mainly of wood shavings, feathers, and droppings, is widely used as an agricultural fertilizer worldwide. The period that antimicrobials persist in agricultural soils could present various environmental and public health concerns. Thus, in this research, a method to identify different families of antimicrobials in poultry litter was developed. Results show that HPLC-MS/MS can reliably detect nine different compounds from three families of antimicrobials. This method was used to identify antimicrobials from commercial poultry flocks, providing a valuable and specific tool to monitor these residues in poultry litter prior to its use as an agricultural fertilizer.