Abstract

Gastric cancer (GC) is the leading cause of cancer death worldwide. Therefore, is relevant identify its molecular basis. Recent studies show that Reprimo (RPRM) plays a key role as tumor suppressor gene, causing cell cycle arrest at the G2 to M. It is believed that loss of expression and function of RPRM could be involved in the early stages of GC.RPRM expression was evaluated in GC cell lines: AGS, NCI-N87, SNU-1 and KATO-III by RT-PCR. We verified the méthylation status of the promoter region through bisulfite sequencing.We determined that 75% of the lines studied expressed mRNA level RPRM. In addition was found that the rate of méthylation (methylated CpG v/s non-methylated) in different cell lines studied was 70%.These results suggest that the loss of RPRM expression could be related with high rate of méthylation in the promoter region in some cells line. In the case of NCI-N87, contains high levels of méthylation in promoter region, however was not able to silence the expression of RPRM. These findings suggest that small differences in the level méthylation of CpGs islets play a critical role in the expression of RPRM. Grant Program Fondecyt 1111014.