Abstract

The Helicobacter pylori (Hp) infection leads to gastroduodenal inflammation mediated by reactive oxygen species (ROS) as well as superoxide dismutase (SOD) and cyclooxygenase (COX-2) expression, both events being involved in the development of gastric cancer (GC). We evaluated the protective effect of phytochemicals and antioxidant from natural sources on the production of ROS and the expression of superoxide dismutase (SOD) in CG cells infected with (Hp). METHODS:Extracts of plants were tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. The effects of extracts from plants, H202 and Hp on gastric cancer lines AGS and SNU1, Were evaluated according to cell viability. To evaluated the protective effects of extracts from plants, the cells lines were pre-treated with extracts by 24 h. Then were exposed to H2O2 and Hp and the effect of each treatment on the cell viability, production of ROS and SOD expression was determined by MTS assay, 2’,7’ dichlorodihydrofluorescein diacetate probe test and Western blot analysis, respectively. RESULTS. After 24 h incubation of SNU1 and AGS cells with extracts plants and infected with Hp, the viability was increased and the generation of ROS diminished. Under the same conditions, the expression of SOD was increased. CONCLUSIONS. The results observed in this study may be result from the antioxidative properties in the extracts as flavonoids, tannins, carotenoids, etc., against generation of ROS induced by Hp. Accordingly, these molecules could have an anti-inflammatory role leading to attenuated cell damage by bacterial infection; this mechanism anti-inflammatory require to be elucidated.