Development of a SYBR Green quantitative polymerase chain reaction assay for rapid detection and quantification of infectious laryngotracheitis virus

Mahmoudian, Alireza; Kirkpatrick, Naomi C.; Coppo, Mauricio; Lee, Sang-Won; Devlin, Joanne M.; Markham, Philip F.; Browning, Glenn F.; Noormohammadi, Amir H.

Abstract

Infectious laryngotracheitis is an acute viral respiratory disease of chickens with a worldwide distribution. Sensitive detection of the causative herpesvirus is particularly important because it can persist in the host at a very low copy number and be transmitted to other birds. Quantification of viral genome copy number is also useful for clinical investigations and experimental studies. In the study presented here, a quantitative polymerase chain reaction (qPCR) assay was developed using SYBR Green chemistry and the viral gene UL15a to detect and quantify infectious laryngotracheitis virus (ILTV) in ILTV-inoculated chicken embryos or naturally infected birds. The specificity of the assay was confirmed using a panel of viral and bacterial pathogens of poultry. The sensitivity of the assay was compared with two conventional PCR assays, virus titration and an antigen-detecting enzyme-linked immunosorbent assay. The qPCR developed in this study was highly sensitive and specific, and has potential for quantification of ILTV in tissues from naturally and experimentally infected birds and embryos.

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Título según WOS: ID WOS:000291806100002 Not found in local WOS DB
Título de la Revista: AVIAN PATHOLOGY
Volumen: 40
Número: 3
Editorial: TAYLOR & FRANCIS LTD
Fecha de publicación: 2011
Página de inicio: 237
Página final: 242
DOI:

10.1080/03079457.2011.553582

Notas: ISI