Electrochemical nucleic acid biosensors for the detection of interaction between peroxynitrite and DNA

De La Fuente E.; Villagra, G; Bollo, S

Abstract

We studied the reactivity of peroxynitrite and different nucleic acid molecules using DNA electrochemical biosensors. SIN-1 (3-morpholinosydnonimine) has been used for the simultaneous generation of NO · and superoxide, i.e., as a peroxynitrite (ONOO -) donor. Double strand DNA (dsDNA), single strand DNA (ssDNA) and 15 guanine bases oligonucleotide (Oligo(dG) 15) were immobilized on a carbon paste electrode to generate the biosensor and DPV was selected as the electroanalytical technique. Results showed that electrochemical biosensors were very sensitive for detecting interaction between ONOO - and DNA. A down/up effect was observed, i.e., at low ONOO - concentrations the guanine oxidation signal decreased while at high ONOO - concentrations the guanine oxidation current increased. Oligo(dG) 15 exhibited greater interaction at low ONOO - concentrations than the other DNA molecules. The reactivity between ONOO - and DNA was also evaluated in solution phase, showing the same down/up effect. Finally, the capacity of DNA to hybridize was prevented after interaction with ONOO -. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

Más información

Título según WOS: Electrochemical nucleic acid biosensors for the detection of interaction between peroxynitrite and DNA
Título según SCOPUS: Electrochemical nucleic acid biosensors for the detection of interaction between peroxynitrite and DNA
Título de la Revista: ELECTROANALYSIS
Volumen: 19
Número: 14
Editorial: WILEY-V C H VERLAG GMBH
Fecha de publicación: 2007
Página de inicio: 1518
Página final: 1523
Idioma: English
URL: http://doi.wiley.com/10.1002/elan.200703885
DOI:

10.1002/elan.200703885

Notas: ISI, SCOPUS