Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi

Galindo, M; Sabaj V; Espinoza, I; Toro, GC; Bua, J.; Grenet, J; Lopez-Solis, R; Ruiz, AM; Galanti N.

Abstract

The Trypanosoma cruzi karyotype shows an extensive chromosomal size polymorphism. Absence of condensed mitotic chromosomes and chromatin fragility are characteristic features of T. cruzi which would allow DNA breaks and chromosomal rearrangements during cell proliferation. We have investigated by pulsed field gel electrophoresis (PFGE) eventual changes in chromosomal size during exponential and stationary phases of T. cruzi epimastigotes in culture, in G0 trypomastigotes and throughout the cell cycle in synchronized epimastigotes. T. cruzi molecular karyotype was stable throughout the cell cycle and during differentiation. Thus, the chromosomal size polymorphism previously reported in T. cruzi contrasts with the stability of the molecular karyotype observed here and suggests that chromosomal rearrangements leading to changes in chromosomal size are scarce events during the clonal propagation of this parasite. © 2007 Federation of European Biochemical Societies.

Más información

Título según WOS: Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi
Título según SCOPUS: Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi
Título de la Revista: FEBS LETTERS
Volumen: 581
Número: 10
Editorial: Elsevier
Fecha de publicación: 2007
Página de inicio: 2022
Página final: 2026
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S001457930700422X
DOI:

10.1016/j.febslet.2007.03.093

Notas: ISI, SCOPUS