Relationship between Helicobacter pylori virulence factors and regulatory cytokines as predictors of clinical outcome

Serrano, C.; Diaz, MI; Valdivia, A; Godoy A.; Peña A; Rollan, A; Kirberg, A.; Hebel, E.; Fierro, J; Klapp, G.; Venegas A.; Harris, PR

Abstract

Helicobacter pylori infection is highly prevalent in Chile (73%). Usually a minority of infected patients develops complications such as ulcers and gastric cancer that have been associated with the presence of virulence factors (cagA, vacA) and host T helper response (Th1/Th2). Our aim was to evaluate the relationship between strain virulence and host immune response, using a multiple regression approach for the development of a model based on data collected from H. pylori infected patients in Chile. We analyzed levels of selected cytokines determined by ELISA (interleukin (IL)-12, IL-10, interferon (IFN)-γ and IL-4) and the presence of cagA and vacA alleles polymorphisms determined by PCR in antral biopsies of 41 patients referred to endoscopy. By multiple regression analysis we established a correlation between bacterial and host factors using clinical outcome (gastritis and duodenal ulcer) as dependent variables. The selected model was described by: clinical outcome = 0.867491 (cagA) + 0.0131847 (IL-12/IL-10) + 0.0103503 (IFN-γ/IL-4) and it was able to explain over 90% of clinical outcomes observations (R2 = 96.4). This model considers that clinical outcomes are better explained by the interaction of host immune factors and strain virulence as a complex and interdependent mechanism. © 2007 Elsevier Masson SAS. All rights reserved.

Más información

Título según WOS: Relationship between Helicobacter pylori virulence factors and regulatory cytokines as predictors of clinical outcome
Título según SCOPUS: Relationship between Helicobacter pylori virulence factors and regulatory cytokines as predictors of clinical outcome
Título de la Revista: MICROBES AND INFECTION
Volumen: 9
Número: 4
Editorial: Elsevier
Fecha de publicación: 2007
Página de inicio: 428
Página final: 434
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S128645790700024X
DOI:

10.1016/j.micinf.2006.12.012

Notas: ISI, SCOPUS