Copeptin, derived from isotocin precursor, is a probable prolactin releasing factor in carp

Flores, CM; Muñoz D; Soto M.; Kausel G.; Romero A.; Figueroa J.

Abstract

Control of prolactin (PRL) release is of crucial importance for the multiple functions exerted by PRL in vertebrates. Recently identified hypothalamic PRL-releasing peptides displayed additional neuromodulatory activities and in fish only few could be detected close to lactotrophs. Here we describe the C-terminal peptide processed from the carp isotocin precursor as probable physiologically relevant regulator of PRL release in carp. The amino acid sequence derived from the complete isotocin precursor gene of Cyprinus carpio, predicted a C-terminal peptide uncleaved between the neurophysin (Np) and copeptin (Cp) domain. Accordingly, antibodies against synthetic Np- and Cp-specific oligopeptides both immunodetected a 13 kDa protein (cNpCp) in total pituitary proteins and showed abundant immunoreaction in hypothalamic axons in direct contact with lactotrophs in the rostral pars distalis of carp pituitary gland sections. Finally, incubation of cultured carp pituitary explants with purified carp cNpCp resulted in a potent stimulation of PRL release. © 2006 Elsevier Inc. All rights reserved.

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Título según WOS: Copeptin, derived from isotocin precursor, is a probable prolactin releasing factor in carp
Título según SCOPUS: Copeptin, derived from isotocin precursor, is a probable prolactin releasing factor in carp
Título de la Revista: GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volumen: 150
Número: 2
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2007
Página de inicio: 343
Página final: 354
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S001664800600298X
DOI:

10.1016/j.ygcen.2006.09.005

Notas: ISI, SCOPUS