Abstract

Background:Antibiotic-dependent pathogenic bacteria are sporadically isolated from patients that received prolonged antibiotic treatments. Evolution of antibiotics dependence and its clinical implications are scarcely studied.Materials methods:A linezolid-dependentStaphylococcus aureusstrain was isolated from a cystic fibrosis patient. A draft genome sequence was obtained and searched for known antibiotics resistance determinants and virulence factors.Results:The genome was assembled into 79 contigs for a total of 2.83 Mbp. This strain is a sequence type 5 methicillin-resistantStaphylococcus aureuswith a type I SCCmeccassette also conserving the Panton-Valentine leukocidin. The G2576T substitution, conferring linezolid resistance, was harbored by all five copies of the 23S rRNA.Conclusion:The linezolid-dependent strain is related to a strain circulating in Latin America that acquired a mutation conferring linezolid resistance.