Identification of Isoquinoline Alkaloids from Berberis Microphylla by HPLC ESI-MS/MS

Manosalva, L.; Mutis, A.; Díaz, J.; Urzúa, A.; Fajardo, V; Quiroz, A.

Keywords: Berberis microphylla, Patagonia, isoquinoline alkaloids, HPLC ESI-MS/MS

Abstract

Berberis microphylla (G. Forst) is a native plant growing in Patagonia. In recent years Patagonia Berberis are becoming important due to their interesting biological properties related to their alkaloids content. The aim of this study was determine the distribution and proportion of isoquinoline alkaloids in leaves, stems and roots of B. microphylla collected in two different climatic zones from Chilean Patagonia. Using by HPLC ESI-MS/MS isocorydine, jatrorrhizine, palmatine, reticuline, scoulerine, tetrahydroberberine and thalifendine were detected for the first time in this specie, and the presence of allocryptopine, berberine, calafatine and protopine, previously isolated in B. microphylla was corroborated. The alkaloids profile showed differences of compounds in samples collected in two climatic zones, where more compounds were detected in plants from Lago Deseado than Cerro Sombrero. Furthermore, a greater number of alkaloids were found in stem and root extracts and berberine and thalifendine were detected in higher proportion in these structures.

Más información

Título de la Revista: BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS
Volumen: 13
Número: 4
Fecha de publicación: 2014
Página de inicio: 324
Página final: 335
Idioma: Inglés