Abstract

In the present study, the taxonomic position of Bacillus tepidiphilus was re-evaluated. Bacillus tepidiphilus (B. tepidiphilus) SYSU G01002(T) showed the highest 16S rRNA gene sequence similarity with the type strain Peribacillus alkalitolerans (P. alkalitolerans) (97.7%). In the phylogenetic (based on 16S rRNA sequence) and phylogenomic (based on 71 bacterial single-copy genes) trees, B. tepidiphilus SYSU G01002(T) clade with the members of the genus Peribacillus. The amino acid identity (AAI) value of B. tepidiphilus SYSU G01002(T) was highest with P. alkalitolerans KCTC 33631(T) (73.6%). The AAI value between B. tepidiphilus SYSU G01002(T) and P. alkalitolerans KCTC 33631(T) was above the cutoff level for genus delineation. The average nucleotide identity (ANI) between B. tepidiphilus and P. alkalitolerans KCTC 33631(T) was 74.1%, which was below the ANI value (95-96%) for species delineation. Based on the phylogenetic, phylogenomic, AAI, and ANI analysis, Bacillus tepidiphilus is proposed to transfer to the genus Peribacillus as Peribacillus tepidiphilus comb. nov. The type strain is SYSU G01002(T) (= KCTC 43131(T) = CGMCC 1.17491(T)).