Abstract

In the present study, the taxonomic position of Bacillus lacisalsi YSP-3(T) was evaluated using phylogenetic and genome-based comparison. B. lacisalsi YSP-3(T) showed the highest 16S rRNA gene sequence similarity to Alteribacter natronophilus M30(T) (98.4%), followed by Alteribacter aurantiacus K1-5(T) (97.5%) and Alteribacter populi FJAT-45347(T) (97.2%). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 71 bacterial single-copy genes) trees, B. lacisalsi YSP-3(T) clustered with the members of the genus Alteribacter. The amino acid identity (AAI) values between B. lacisalsi YSP-3(T) and the members of the genus Alteribacter were >65%, which is above the cut-off level (65-95%) for genus delineation. The average nucleotide identity (ANI) values between B. lacisalsi YSP-3(T) and the members of the genus Alteribacter were 95%, which is lower than the threshold value (95-96%) for bacterial species delineation. The AAI value suggested that B. lacisalsi YSP-3(T) was a member of the genus Alteribacter while the ANIb value suggested it as a novel species of the genus Alteribacter. Based on the results, we propose to transfer Bacillus lacisalsi to the genus Alteribacter as Alteribacter lacisalsi comb. nov.