Cooperative action of attached and planktonic cells during bioleaching of chalcopyrite with Sulfolobus metallicus at 70 degrees C

Gautier, V; Escobar B.; Vargas, T.

Abstract

The catalytic influence of Sulfolobus metallicus in the bioleaching of chalcopyrite at 70 °C and pH 1.5 was studied in shake flask experiments. Leaching was conducted in an iron-free basal medium with a - 80 # + 120 # chalcopyrite sample, and was characterized from monitoring in solution the pH, Eh, copper and iron concentration, and cell population. In order to evaluate separately the influence of planktonic and attached cells on the catalytic process, in some experiments the microorganisms were prevented from reaching the chalcopyrite surface by keeping them in a chamber separated by a 0.1 Millipore membrane. In a complementary experimental series, the concentration of different sulfur species dissolved in the solution was determined with HPLC at different conditions, under nitrogen or air, abiotic or inoculated with S. metallicus. Maximum copper dissolution was reached when at least one fraction of the inoculated microorganisms were able to approach chalcopyrite surface. In this case, notably, the fraction of the population of microorganisms that were prevented from reaching chalcopyrite was still observed to increase. In experiments where all the inoculated microorganisms were prevented from reaching chalcopyrite, the cell population did not grow. In this case copper dissolution was only 50% of the amount reached when full bacterial contact was allowed, and was similar to the amount of copper dissolved at aerated-abiotic conditions. HPLC results on the analysis of dissolved sulfur species showed that when microorganisms were able to reach chalcopyrite there was accumulation in solution of thiosulfate (S2O3)- 2 and sulfite (SO3)- 2, in addition to the formation of bisulfite (HSO3)-, bisulfate (HSO4)- and sulfate (SO4)- 2 which was also observed in aerobic-abiotic conditions. It could be concluded that in bioleaching of chalcopyrite in the presence of S. metallicus there is a cooperative action between attached cells which can oxidize sulfur-containing surface layers on chalcopyrite, forming thiosulfate, sulfite and bisulfite, and planktonic cells which further oxidize these intermediate compound to bisulfate and sulfate. Removal of surface passivating layers under the catalytic action of attached microorganisms is a key catalytic factor as it greatly enhances the oxidative action of ferric iron on chalcopyrite. © 2008 Elsevier B.V. All rights reserved.

Más información

Título según WOS: Cooperative action of attached and planktonic cells during bioleaching of chalcopyrite with Sulfolobus metallicus at 70 degrees C
Título según SCOPUS: Cooperative action of attached and planktonic cells during bioleaching of chalcopyrite with Sulfolobus metallicus at 70 °C
Título de la Revista: HYDROMETALLURGY
Volumen: 94
Número: 01-abr
Editorial: Elsevier
Fecha de publicación: 2008
Página de inicio: 121
Página final: 126
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0304386X08002028
DOI:

10.1016/j.hydromet.2008.05.036

Notas: ISI, SCOPUS