Human branching cholangiocyte organoids functional bile duct formation

Roos, Floris J. M.; van Tienderen, Gilles S.; Wu, Haoyu; Bordeu, Ignacio; Vinke, Dina; Albarinos, Laura Munoz; Monfils, Kathryn; Niesten, Sabrah; Smits, Ron; Willemse, Jorke; Rosmark, Oskar; Westergren-Thorsson, Gunilla; Kunz, Daniel J.; de Wit, Maurice; French, Pim J.; et. al.

Abstract

Human cholangiocyte organoids show great promise for regenerative therapies and in vitro modeling of bile duct development and diseases. However, the cystic organoids lack the branching morphology of intrahepatic bile ducts (IHBDs). Here, we report establishing human branching cholangiocyte organoid (BRCO) cultures. BRCOs self-organize into complex tubular structures resembling the IHBD architecture. Single-cell transcriptomics and functional analysis showed high similarity to primary cholangiocytes, and importantly, the branching growth mimics aspects of tubular development and is dependent on JAG1/NOTCH2 signaling. When applied to cholangiocarcinoma tumor organoids, the morphology changes to an in vitro morphology like primary tumors. Moreover, these branching cholangiocarcinoma organoids (BRCCAOs) better match the transcriptomic profile of primary tumors and showed increased chemoresistance to gemcitabine and cisplatin. In conclusion, BRCOs recapitulate a complex process of branching morphogenesis in vitro. This provides an improved model to study tubular formation, bile duct functionality, and associated biliary diseases.

Más información

Título según WOS: ID WOS:000804044900001 Not found in local WOS DB
Título de la Revista: CELL STEM CELL
Volumen: 29
Número: 5
Editorial: Cell Press
Fecha de publicación: 2022
Página de inicio: 776
Página final: +
DOI:

10.1016/j.stem.2022.04.011

Notas: ISI