Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells

Celedon, G; Gonzalez G. ; Barrientos, D; Pino, J; Venegas, F; Lissi, EA; Soto C.; Martinez, D.; Alvarez C.; Lanio, ME

Abstract

We have investigated the relationship between the status of red blood cells (RBCs) and their susceptibility to toxin sticholysin II (StII) hemolytic activity; we have evaluated this effect in different RBC ensembles, comprising young and old cells, and in cells partially damaged by their pre-exposition to a free radical source. Upon action of StII, young cell populations are less prone to hemolysis than the whole population, while old cell populations and peroxyl-oxidized red cells are lysed faster than the whole population. Cell K+ content was higher in young cells and lower in both senescent cells and in peroxyl-damaged cells relative to whole cell population. The relevance of cell K+ content in St II-induced lysis was shown when external Na+ was partially replaced by K+; under this condition, RBC lysed faster in the presence of St II but no difference was observed among young cells, whole cells population and peroxyl-damaged cells; only old cells lysed faster that the whole population, response that can be due to an enhanced St II-induced pore formation as supported by evaluation of St II irreversible binding to RBC. It is concluded that this factor and the amount of intracellular K+ are the dominant parameters that modulate the resistance of RBC to St II-induced lysis. © 2008 Elsevier Ltd. All rights reserved.

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Título según WOS: Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells
Título según SCOPUS: Stycholysin II, a cytolysin from the sea anemone Stichodactyla helianthus promotes higher hemolysis in aged red blood cells
Título de la Revista: TOXICON
Volumen: 51
Número: 8
Editorial: PERGAMON-ELSEVIER SCIENCE LTD
Fecha de publicación: 2008
Página de inicio: 1383
Página final: 1390
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0041010108001001
DOI:

10.1016/j.toxicon.2008.03.006

Notas: ISI, SCOPUS