Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis

Leal-Ortiz, S; Waites, CL; Terry-Lorenzo, R; Zamorano P.; Gundelfinger ED; Garner, CC

Abstract

Active zones are specialized regions of the presynaptic plasma membrane designed for the efficient and repetitive release of neurotransmitter via synaptic vesicle (SV) exocytosis. Piccolo is a high molecular weight component of the active zone that is hypothesized to participate both in active zone formation and the scaffolding of key molecules involved in SV recycling. In this study, we use interference RNAs to eliminate Piccolo expression from cultured hippocampal neurons to assess its involvement in synapse formation and function. Our data show that Piccolo is not required for glutamatergic synapse formation but does influence presynaptic function by negatively regulating SV exocytosis. Mechanistically, this regulation appears to be calmodulin kinase II-dependent and mediated through the modulation of Synapsin1a dynamics. This function is not shared by the highly homologous protein Bassoon, which indicates that Piccolo has a unique role in coupling the mobilization of SVs in the reserve pool to events within the active zone. © 2008 Leal-Ortiz et al. The Rockefeller University Press.

Más información

Título según WOS: Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis
Título según SCOPUS: Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis
Título de la Revista: JOURNAL OF CELL BIOLOGY
Volumen: 181
Número: 5
Editorial: ROCKEFELLER UNIV PRESS
Fecha de publicación: 2008
Página de inicio: 831
Página final: 846
Idioma: English
URL: http://www.jcb.org/cgi/doi/10.1083/jcb.200711167
DOI:

10.1083/jcb.200711167

Notas: ISI, SCOPUS