Microribonucleic acid-21 increases aldosterone secretion and proliferation in H295R human adrenocortical cells

Romero, DG; Plonczynski, MW; Carvajal, CA; Gomez-Sanchez, EP; Gomez-Sanchez, CE

Abstract

MicroRNAs (miRNAs) are endogenous small noncoding RNAs that decrease the expression levels of specific genes by translational repression, sequestration, and degradation of their mRNAs. Angiotensin II is an important modulator of adrenal zona glomerulosa cell physiology, including steroidogenesis and proliferation among many other physiological processes. Because each miRNA may regulate the expression levels of multiple genes, thereby resembling the transcription regulatory networks triggered by transcription factors, we hypothesize that specific miRNAs may be involved in angiotensin II-mediated adrenocortical cell physiology. The human adrenocortical cell line H295R is the only adrenal cell line available with a steroid secretion pattern and regulation similar to freshly isolated adrenocortical cells. We screened for miRNAs regulated by angiotensin II in H295R cells and found that miRNA-21 expression levels were specifically modulated by angiotensin II. Angiotensin II time dependently increased miRNA-21 expression reaching a 4.4-fold induction after 24 h. Angiotensin II-mediated miRNA-21 expression resulted in biologically active miRNA-21, determined using a fusion mRNA reporter system carrying miRNA-21 target sequences in its 3′ untranslated region. Up-regulation of miRNA-21 intracellular levels increased aldosterone secretion but not cortisol. Elevation of miRNA-21 levels also increased cell proliferation in H295R cells. In summary, miRNA-21 is an endogenously expressed miRNA in human adrenal cells. miRNA-21 expression is up-regulated by angiotensin II, and its overexpression caused an increase in aldosterone secretion and cell proliferation. Alterations in miRNA-21 expression levels or function may be involved in dysregulation of angiotensin II signaling and abnormal aldosterone secretion by adrenal glands in humans. Copyright © 2008 by The Endocrine Society.

Más información

Título según WOS: Microribonucleic acid-21 increases aldosterone secretion and proliferation in H295R human adrenocortical cells
Título según SCOPUS: Microribonucleic acid-21 increases aldosterone secretion and proliferation in H295R human adrenocortical cells
Título de la Revista: ENDOCRINOLOGY
Volumen: 149
Número: 5
Editorial: OXFORD UNIV PRESS INC
Fecha de publicación: 2008
Página de inicio: 2477
Página final: 2483
Idioma: English
URL: http://press.endocrine.org/doi/abs/10.1210/en.2007-1686
DOI:

10.1210/en.2007-1686

Notas: ISI, SCOPUS