Adenosine A(2B) receptor mediates an increase on VEGF-A production in rat kidney glomeruli

Valladares, D; Quezada C.; Montecinos, P; Yañez A.; Sobrevia L.; Martin, RS

Abstract

Up-regulation of the glomerular expression and the activity of vascular endothelial growth factor-A (VEGF) have been identified as an early pathogenic event for the progression of diabetic nephropathy. Currently, however the mediators are not yet clearly recognized. In this study we identified all four adenosine receptor (AR) subtypes, i.e. A1, A2A, A2B and A3 in isolated rat kidney glomeruli. We localized the expression of A2BAR in podocytes, the primary VEGF producing cells. The ex vivo treatment of kidney glomeruli with adenosine or a general AR agonist NECA, increases VEGF protein content. In addition, NECA treatment elicits VEGF release. These effects were blocked by the A2BAR selective antagonist MRS1754 supplementation. Furthermore, we showed that A2BAR activation was necessary to promote a higher expression of VEGF in kidney glomeruli upon exposure to high d-glucose concentration, a pathogenic condition like those observed in diabetic nephropathy. © 2007 Elsevier Inc. All rights reserved.

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Título según WOS: Adenosine A(2B) receptor mediates an increase on VEGF-A production in rat kidney glomeruli
Título según SCOPUS: Adenosine A2B receptor mediates an increase on VEGF-A production in rat kidney glomeruli
Título de la Revista: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volumen: 366
Número: 1
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2008
Página de inicio: 180
Página final: 185
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0006291X0702534X
DOI:

10.1016/j.bbrc.2007.11.113

Notas: ISI, SCOPUS