Abstract

The present invention relates to a method for detecting Mycobacterium bovis based on fluorescent semiconductor nanoparticle nano-genosensors (Quantum Dots, QDs), genomic probes and quencher. Upon exposure of the nano-genosensor to M. bovis DNA, the quencher is displaced, and the DNA binds to the probe bound to the fluorescent semiconductor nanoparticle composed of Cadmium Telluride (CdTe), causing an increase in fluorescence (turn-on). The photonic nano-genosensor emits fluorescence proportional to the amount of Mycobacterium bovis DNA present in a sample.