Abstract

Strain T-12T, T , an orange, Gram- stain- negative, non- motile, rod- shaped strain, was isolated in November 2013 from water samples collected from an Atlantic salmon ( Salmo salar) ) fry culturing system at a fish farm in Chile. Phylogenetic analysis based on 16S rRNA sequences (1394 bp) revealed that strain T- 12T T belonged to the genus Flavobacterium, , showing close relationships to Flavobacterium bernardetii F-372T T (99.48 %) and Flavobacterium terrigena DS-20T T (98.50 %). The genome size of strain T-12T T was 3.28 Mb, with a G+C content of 31.1 mol%. Genome comparisons aligned strain T- 12T T with Flavobacterium bernardetii F-372T T (GCA_011305415) and Flavobacterium terrigena DSM 17934T T (GCA_900108955). The highest digital DNA-DNA hybridization (dDDH) values were 42.6 % with F. bernardetii F-372T T (GCA_011305415) and 33.9 % with F. terrigena DSM 17934T T (GCA_900108955). Pairwise average nucleotide identity (ANI) calculations were below the species cutoff, with the best results with F. bernardetii F-372T T being: ANIb, 90.33 %; ANIm, 91.85 %; and TETRA, 0.997 %. These dDDH and ANI results confirm that strain T-12T T represents a new species. The major fatty acids were iso- C15: 15: 0 and C 15:1 omega 6 & scy;. Detected polar lipids included phospholipids (n=2), n =2), aminophospholipid (n=1), n =1), aminolipid (n=1) n =1) and unidentified lipids (n=2). n =2). The predominant respiratory quinone was menaquinone MK7 (80 %) followed by MK- 6 (20 %). Phenotypic, chemotaxonomic, and genomic data support the classification of strain T-12T T (=CECT 30410T=RGM T =RGM 3222T) T ) as representing a novel species of Flavobacterium, , for which the name Flavobacterium facile sp. nov. is proposed.