Article ISI SCOPUS
BIOORGANIC MEDICINAL CHEMISTRY LETTERS  (2009)

Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals

Aspée A.; Orrego A; Alarcon, E.; Lopez-Alarcon, C; Poblete, H.; Gonzalez-Nilo, D

Abstract

A new strategy to evaluate accessibility of antioxidants to radical proteins has been developed using nitroxide prefluorescent probes anchored into human serum albumin (HSA). Binding association constants for the nitroxide probes C343T and QT with HSA were 5 × 104 and 9 × 104 M-1, respectively. Rate constants for the nitroxide reduction by antioxidants in HSA were determined finding kHSA/kbuffer ratio of 0.8, 1.9, and 0.075 for ascorbic acid, Trolox, and caffeic acid, respectively, for the nitroxide C343T reduction. © 2009 Elsevier Ltd. All rights reserved.

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Título según WOS: Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals
Título según SCOPUS: Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals
Título de la Revista: BIOORGANIC MEDICINAL CHEMISTRY LETTERS
Volumen: 19
Número: 22
Editorial: PERGAMON-ELSEVIER SCIENCE LTD
Fecha de publicación: 2009
Página de inicio: 6382
Página final: 6385
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0960894X09013389
DOI:

10.1016/j.bmcl.2009.09.070
Notas: ISI, SCOPUS