Abstract

Nanoemulsions (NEs) offers a viable approach to enhance the solubility and bioavailability of poorly soluble natural compounds with therapeutic potential by reducing their particle size to nanoscale (1). A reducing their particle size to the nanoscale allow to increase the solubility and enhancing absorption and biodistribution in the target tissues (2,3). The aim of this study was to develop a jojoba oil-based NE as a nanosized dispersion for transporting insoluble bioactive compounds, including polyphenols and phenolic compounds such as t-resveratrol and curcuminoids, among others. Various formulations were created by blending water, jojoba oil, Isopropyl myristate and a Tween 80/Span 20 Mixture (Surfmix). Polyphenol-enriched extract (PPE - highly purified), serving as a model for poorly soluble drugs, was dissolved in jojoba oil. Formulations with varying oil to Surfmix were then dispersed in water using ultrasonic homogenization to achieve nanosized droplets, leveraging the cavitation principle. These formulations were characterized by dynamic light scattering (DLS). Selected formulations underwent antimicrobial and cytoxicity evaluations to ensure the manufacturing process did not diminish the therapeutic efficacy of the bioactive. The impact on cell viability was assessed using an MTT assay on Adult Dermal Fibroblasts (HDFa), and antimicrobial effectiveness was tested against Staphylococcus aureus in planktonic model.