Is Single-Strand Conformation Polymorphism Analysis of the Full 5 ' Untranslated Region an Adequate Approach To Study Hepatitis C Virus Quasispecies Distribution?

Vera-Otarola, J; Barría MI; León U; Carvallo, P.; Soza A.; Lopez-Lastra, M

Abstract

Single-strand conformation polymorphism (SSCP) analysis is used by many laboratories to study the quasispecies distribution of the hepatitis C virus (HCV). Here we question the validity of this experimental approach, as conclusions are drawn from the analysis of the migration patterns of two ssDNA molecules and not from RNA. Using previously characterized mutants of the HCV 5' untranslated regions, we show that contrary to what has been predicted, SSCP migration patterns of DNA amplicons with differences in their nucleotide sequences generated from the full 5' UTR of HCV are not necessarily unique. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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Título según WOS: Is Single-Strand Conformation Polymorphism Analysis of the Full 5 ' Untranslated Region an Adequate Approach To Study Hepatitis C Virus Quasispecies Distribution?
Título según SCOPUS: Is single-strand conformation polymorphism analysis of the full 5? untranslated region an adequate approach to study hepatitis C virus quasispecies distribution?
Título de la Revista: JOURNAL OF VIROLOGY
Volumen: 83
Número: 17
Editorial: AMER SOC MICROBIOLOGY
Fecha de publicación: 2009
Página de inicio: 9018
Página final: 9021
Idioma: English
URL: http://jvi.asm.org/cgi/doi/10.1128/JVI.00971-09
DOI:

10.1128/JVI.00971-09

Notas: ISI, SCOPUS