Abstract

Juvenile northern Chilean scallops of 937±55 µm shell height were exposed to five different concentrations of chloramphenicol (CHL) (5, 10, 25, 50 and 100 µg mL-1), plus a control without antibiotics. To determine the effect of CHL on the accompanying microflora, the number of colony-forming units (CFU) that grew on TGE culture medium was counted in the seawater of containers with juveniles, and in containers with microalgae used as food. Both were exposed to the same concentrations of CHL. The growth rates of juveniles treated with CHL and the control without antibiotic showed highly significant differences (P=0.0001). The growth rate was inversely proportional to the CHL concentration. The control sample presented the highest growth rate (84.4±14.3 µm day-1), followed by the sample treated with 5 µg mL-1 (64.2±14.3 µm day-1). The survival in the control and in the treated samples with 5-50 µg mL-1 was rather high, with a mean value of 95%. Only the sample treated with 100 µg mL-1 had a low survival (36.7%). The CFU count was larger in the containers with juveniles, when compared with the ones with food. The CFU count tended to decrease with increasing CHL concentration in the juveniles. © 2009 Blackwell Publishing Ltd.