Plasmodium falciparum: Effect of Solanum nudum steroids on thiol contents and beta-hematin formation in parasitized erythrocytes

Pabon, A; Deharo, E; Zuluaga, L; Maya, JD; Saez, J; Blair, S

Abstract

We studied the effects on total thiols glutathione (GSH) and cysteine contents in Plasmodium falciparum in vitro when treated with four steroid derivatives and a sapogenin (Diosgenone) extracted from Solanum nudum. We also determined their capacity to inhibit ß-hematin formation. We showed that SN-1 (16a-acetoxy-26-hydroxycholest-4-ene-3,22-dione) increased total glutathione and cysteine concentrations while SN-4 (26-O-ß-d-glucopyranosyloxy-16a-acetoxycholest-4-ene-3,22-dione) decreased the concentration of both thiols. Acetylation in C16 was crucial for the effect of SN-1 while type furostanol and terminal glucosidation were necessary for the inhibitory properties of SN-4. The combination of steroids and buthionine sulfoximine, a specific inhibitor of a step-limiting enzyme in GSH synthesis, did not modify the glutathione contents. Finally, we found that SN-1 inhibited more than 80% of ß-hematin formation at 5.0 mM, while the other steroids did not show any effect. © 2009 Elsevier Inc. All rights reserved.

Más información

Título según WOS: Plasmodium falciparum: Effect of Solanum nudum steroids on thiol contents and beta-hematin formation in parasitized erythrocytes
Título según SCOPUS: Plasmodium falciparum: Effect of Solanum nudum steroids on thiol contents and ?-hematin formation in parasitized erythrocytes
Título de la Revista: EXPERIMENTAL PARASITOLOGY
Volumen: 122
Número: 4
Editorial: ACADEMIC PRESS INC ELSEVIER SCIENCE
Fecha de publicación: 2009
Página de inicio: 273
Página final: 279
Idioma: English
URL: http://linkinghub.elsevier.com/retrieve/pii/S0014489409001234
DOI:

10.1016/j.exppara.2009.04.014

Notas: ISI, SCOPUS