Oxidation of 5-hydroxymethylfurfural to 5- formyl-2-furancarboxylic acid catalyzed by laccase in a multiphasic gas-liquid microbioreactor
Abstract
Lignocellulosic residues offer a compelling alternative to produce furans such as 5-hydroxymethylfurfural (HMF), a precursor that can be further oxidized to yield 2,5-furandicarboxylic acid (FDCA). The oxidation of HMF to FDCA, catalyzed by the laccase enzyme using TEMPO as a mediator, presents a fascinating alternative for FDCA. A notable feature of its reaction mechanism is the formation of the intermediate compound 5-formyl-2-furancarboxylic acid (FFCA), which subsequently undergoes oxidation to yield FDCA. FFCA has potential applications in medicine and as a base for the manufacture of polyester, it has been little reported by chemical and biochemical synthesis. Due to its attractive properties, the purpose of this work was to assess for the first time the bio-oxidation of HMF to produce FFCA catalyzed by Trametes versicolor laccase in a multiphasic flow microbioreactor. The oxidation of HMF to FFCA was studied in a batch bioreactor and microbioreactors with different internal diameters (ID). Using microbioreactors, the effect of the flow rate of the liquid and gas phase on the yield, space time yield (STYFFCA), and gas–liquid mixture velocity (UM) of the reaction was evaluated. The biooxidation in flow microbioreactors, a selectivity of 100 % for FFCA was achieved, while with the batch bioreactor at the same substrate concentration a selectivity of 6.7 % was obtained. The highest yield (30 %) with 15 mM of 5-hydroxymethylfurfural (HMF) was reached at a gas–liquid flow rate of 0.5 µL/min and the highest STYFFCA (0.07 mol m− 3 min− 1) was achieved at a gas–liquid flow rate of 1.5 µL/min with the microbioreactor with an ID of 0.5 mm. The UM values (0.5 to 1.6 cm min-1) indicated that the reaction takes place under a kinetic regime without mass transfer limitations
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Fecha de publicación: | 2024 |
Año de Inicio/Término: | octubre 2024 |