Dendritic Assembly of Heteromeric gamma-Aminobutyric Acid Type B Receptor Subunits in Hippocampal Neurons

Ramirez, OA; Vidal, RL; Tello, JA; Vargas, KJ; Kindler, S; Haertel, S.; couve, a

Abstract

Understanding the mechanisms that control synaptic efficacy through the availability of neurotransmitter receptors depends on uncovering their specific intracellular trafficking routes. ?-Aminobutyric acid type B (GABAB) receptors (GABABRs) are obligatory heteromers present at dendritic excitatory and inhibitory postsynaptic sites. It is unknown whether synthesis and assembly of GABABRs occur in the somatic endoplasmic reticulum (ER) followed by vesicular transport to dendrites or whether somatic synthesis is followed by independent transport of the subunits for assembly and ER export throughout the somatodendritic compartment. To discriminate between these possibilities we studied the association of GABABR subunits in dendrites of hippocampal neurons combining live fluorescence microscopy, biochemistry, quantitative colocalization, and bimolecular fluorescent complementation. We demonstrate that GABABR subunits are segregated and differentially mobile in dendritic intracellular compartments and that a high proportion of non-associated intracellular subunits exist in the brain. Assembled heteromers are preferentially located at the plasma membrane, but blockade of ER exit results in their intracellular accumulation in the cell body and dendrites. We propose that GABABR subunits assemble in the ER and are exported from the ER throughout the neuron prior to insertion at the plasma membrane. Our results are consistent with a bulk flow of segregated subunits through the ER and rule out a post-Golgi vesicular transport of preassembled GABABRs. © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

Más información

Título según WOS: Dendritic Assembly of Heteromeric gamma-Aminobutyric Acid Type B Receptor Subunits in Hippocampal Neurons
Título según SCOPUS: Dendritic assembly of heteromeric ?-aminobutyric acid type B receptor subunits in hippocampal neurons
Título de la Revista: JOURNAL OF BIOLOGICAL CHEMISTRY
Volumen: 284
Número: 19
Editorial: Elsevier
Fecha de publicación: 2009
Página de inicio: 13077
Página final: 13085
Idioma: English
URL: http://www.jbc.org/cgi/doi/10.1074/jbc.M900575200
DOI:

10.1074/jbc.M900575200

Notas: ISI, SCOPUS