Abstract

The ice plant (Mesembryanthemum crystallinum L.) is a halophyte that could become an alternative crop because of its interest as a functional food and its adaptation to high-saline soils. In this work, leaves from wild ice plants were compared with their cultivated counterparts in a soilless system at different salinities and light exposures for assessing growth parameters, moisture, fatty acid profiles, total carotenoids, phenolic compounds, vitamin C, antioxidant activity, and antiproliferative activity against the HT-29 colorectal cancer cell line. Moisture ranged between 876 and 955 g kg−1, and wild plants contained higher proportions of α-linolenic acid (58.7%–60.7% of total fatty acids) than cultivated ones (20.4%–36.6%). Vitamin C ranged between 819 and 1143 mg kg−1 fresh leaves. Higher salinity led to a larger production of carotenoids, whereas plant mass, total phenolic content, and antioxidant activity increased in plants grown using L8 NS1 and L8 AP67 lamps in comparison with white-light ones. Phenolic profiles were assessed by LC coupled to a hybrid mass spectrometer Q-Orbitrap. Total phenolic acid content was 3–4-fold higher than that of flavonoids, and sinapic, p-coumaric, gallic, 4-hydroxybenzoic, and 2-hydroxy-4-methoxybenzoic acids, as well as gallocatechin, occurred in all samples. Hydroalcoholic extracts of ice plant leaves showed dose- and time-dependent antiproliferative activity against the HT-29 human colorectal cancer cell line, and GI50 was between 920 and 977 μg mL−1 of plant extract. This work contributes to improving knowledge about the growth parameters, phytochemical profiles, and biological activities of wild and cultivated ice plants.