Abstract

Background and aims: Platelets and inflammation play crucial roles in the atherothrombotic process of acute myocardial infarction (AMI), particularly in ischemia/reperfusion injury. This study aims to characterize the pro-inflammatory content of the platelet secretome in AMI patients, assess its effect on nitric oxide (NO) production, and correlate these findings with clinical parameters. Methods: Blood samples from 20 AMI patients and 20 controls were analyzed. Platelets were isolated and stimulated with thrombin, and their secretomes were collected. Endothelial cells were exposed to these secretomes, and NO production was measured. Our results demonstrate a significant reduction in NO production in endothelial cells exposed to the AMI platelet secretome (p < 0.05), suggesting acute endothelial dysfunction. Results: Elevated levels of interleukin-6 (IL-6) (p = 0.0323) and interleukin-8 (IL-8) (p = 0.0197) were identified in the secretome of AMI patients, correlating with clinical markers of myocardial injury. Specifically, IL-6 positively correlated with CK-MB levels (p = 0.04, Pearson r = 0.53), while IL-8 levels inversely correlated with NO production (p = 0.0141, Pearson r = -0.5652). Conclusions: These findings underscore the critical role of platelet-derived IL-6 and IL-8 in endothelial dysfunction and myocardial injury in AMI patients. Future studies should explore the interactions between these cytokines in endothelial cells to further elucidate their roles in AMI pathology.