Abstract

This study focuses on the collection and analysis of hemolymph from adult spiders belonging to the genus Grammostola, specifically those marketed as G. porteri. Using the cardiac puncture technique, hemolymph was obtained without causing permanent harm to the arachnids, representing an ethical alternative to traditional extraction methods that require limb amputation. Morphological results confirmed that the specimens exhibited typical characteristics of the genus Grammostola. The protein components of the hemolymph were analyzed, showing a predominance of bands at 70 and 25 kDa. The recovery hemocytes were mainly granulocytes, and phylogenetic analyses allowed grouping of different families and genera within the suborder Mygalomorphae. Additionally, the implications of the findings on the taxonomy of G. porteri were discussed, corroborating its classification as a synonym of G. rosea. This study not only contributes to the understanding of the diversity and systematics of tarantulas in Chile but also proposes an innovative approach for the collection of genetic material, promoting more sustainable practices in arachnological research.